primary human islets  (BioChain Institute)

Journal: bioRxiv

Article Title: Stress-free Bioprinting of Human Primary and iPSC-derived Islets with Retained Functionality

doi: 10.1101/2024.10.14.617656

Figure Lengend Snippet: A) Schematic view of printing primary cadaveric human islets embedded in a 3% w/v alg/ 6% w/v MC bioink at 500 IEQ: 1 mL bioink. The printed primary islet construct is then cultured for 3-7 days to monitor for functionality and viability. Created using Biorender.com. B) Live Dead staining of printed primary islets 3 days post-printing. All scale bars convey 250 μm. Live cells are shown in green, dead cells are shown in red. C) Percentage of individual printed primary islet represented by viable cells, as indicated by green signal in Live/Dead staining (N=8). D) Location of dead cells present in individual printed primary islet as indicated by red signal in Live Dead staining. r/R of 0.0 indicates that the dead cell is present in the center of the islet, and an r/R of 1.0 indicates that the dead cell is present at the edge of the islet. E) Primary islets were printed and maintained in culture for 7 days. Islets are fluorescently stained for nuclei (DAPI), islet marker c-peptide (shown in green), and islet marker glucagon (shown in red). Scale bar representing 50 μm (*: p < 0.05). F) Glucose stimulated insulin secretion of printed primary islets after 7 days in culture G) Stimulation index from GSIS of second high glucose period normalized to second low glucose period (n=3).

Article Snippet: [ – ] Human cadaveric primary islets (Prodo Labs) were printed in the alginate/methylcellulose ink and maintained in culture for 3 to 7 days ( ).

Techniques: Construct, Cell Culture, Staining, Marker

Journal: EMBO Molecular Medicine

Article Title: Paired box 6 gene delivery preserves beta cells and improves islet transplantation efficacy

doi: 10.15252/emmm.202317928

Figure Lengend Snippet: A, B (A) Islet insulin content and (B) islet apoptosis of normal or T2D human islets with AAV transduction ( n = 8). C, D Representative immunostaining and quantification showing (C) insulin/Ki67 and (D) insulin/TUNEL signals in normal or T2D human islets with AAV transduction ( n = 5–6). Scale bar = 20 μm. E Protein expression of insulin and incretin signaling components in normal or T2D human islets with AAV transduction ( n = 4). F Phosphorylated and total Akt in human islets after 15‐min insulin (100 nM) stimulation ( n = 4). G, H Phosphorylated and total CREB in human islets after 15‐min (G) Exendin‐4 (10 nM) or (H) GIP (10 nM) stimulation ( n = 4). Data information: Each n represents an independent biological replicate (A–H). One‐way ANOVA (A–D). One‐way ANOVA and Kruskal–Wallis test (E). (F–H) Two‐way ANOVA. Data are means ± SEM. ns, nonsignificant. Source data are available online for this figure.

Article Snippet: Primary human islets from seven normal and five T2D donors (Appendix Table ) were obtained from Prodo Laboratories, Inc. (Aliso Viejo, CA, USA).

Techniques: Transduction, Immunostaining, TUNEL Assay, Expressing

Journal: EMBO Molecular Medicine

Article Title: Paired box 6 gene delivery preserves beta cells and improves islet transplantation efficacy

doi: 10.15252/emmm.202317928

Figure Lengend Snippet: A–C (A) Fasting blood glucose, (B) glucose tolerance, and (C) glucose profiles calculated as AUC of control or STZ‐treated mice transplanted with normal or T2D human islets ( n = 7–8). D Serum human insulin ( n = 7–8). E Representative immunostaining showing insulin/glucagon signal and ELISA measurement of human insulin content in human islet grafts ( n = 4–5). Scale bar = 100 μm. F Representative immunostaining and quantification showing insulin/TUNEL signal in human islet grafts ( n = 5). Scale bar = 100 μm. Data information: Each n represents the measurement of a sample from distinct mice (A–F). One‐way ANOVA (A–F). Data are means ± SEM. AUC, area under the curve; ns, nonsignificant. Source data are available online for this figure.

Article Snippet: Primary human islets from seven normal and five T2D donors (Appendix Table ) were obtained from Prodo Laboratories, Inc. (Aliso Viejo, CA, USA).

Techniques: Immunostaining, Enzyme-linked Immunosorbent Assay, TUNEL Assay